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antibodies against cyclin dependent kinase cdk 2  (Proteintech)


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    Structured Review

    Proteintech antibodies against cyclin dependent kinase cdk 2
    Antibodies Against Cyclin Dependent Kinase Cdk 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclin-dependent+kinase+(cdk)2+antibody/CDK2+Antibody/pm41734476-72-0-40
    Average 96 stars, based on 479 article reviews
    antibodies against cyclin dependent kinase cdk 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Carfilzomib inhibits the progression of hepatocellular cancer by upregulating GADD45α expression.
    Article Snippet: Antibodies were procured from Proteintech Group, Inc., including cyclin‐dependent kinase (CDK)2 (cat. no. 10122‐1‐AP), CDK4 (cat. no. 11026‐1‐AP), cyclin A2 (cat. no. 66391‐1‐Ig), cyclin E1 (cat. no. 11554‐1‐AP), GAPDH (cat. no. 60004‐1‐Ig), phosphorylated (p)‐JNK (Tyr185) recombinant antibody (cat. no. 80024‐1‐RR), JNK monoclonal antibody (cat. no. 66210‐1‐Ig), p38 MAPK polyclonal antibody (cat. no. 14064‐1‐AP), p‐p38 MAPK (Thr180/Tyr182) polyclonal antibody (cat. no. 28796‐1‐AP).

    Article Title: Carfilzomib inhibits the progression of hepatocellular cancer by upregulating GADD45α expression
    Article Snippet: Antibodies were procured from Proteintech Group, Inc., including cyclin-dependent kinase (CDK)2 (cat. no. 10122-1-AP), CDK4 (cat. no. 11026-1-AP), cyclin A2 (cat. no. 66391-1-Ig), cyclin E1 (cat. no. 11554-1-AP), GAPDH (cat. no. 60004-1-Ig), phosphorylated (p)-JNK (Tyr185) recombinant antibody (cat. no. 80024-1-RR), JNK monoclonal antibody (cat. no. 66210-1-Ig), p38 MAPK polyclonal antibody (cat. no. 14064-1-AP), p-p38 MAPK (Thr180/Tyr182) polyclonal antibody (cat. no. 28796-1-AP).


    Article Title: ATP-binding cassette subfamily A member 5 suppresses pancreatic ductal adenocarcinoma progression and chemoresistance by promoting β-catenin ubiquitin-dependent degradation.
    Article Snippet: Aims: Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies characterized by poor prognosis and resistance to therapy.. Despite the most widely recognized role of ATP binding cassette (ABC) family transporters in multidrug resistance, emerging evidence suggests that ATP-binding cassette transporter A5 (ABCA5) acts as a tumor suppressor.. Nevertheless, the role and molecular mechanism of ABCA5 in PDAC have not yet been fully investigated.

    Membrane:

    Article Title: CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.
    Article Snippet: Total protein was quantified using a BCA Protein Assay kit (cat. no. P0012; Beyotime Institute of Biotechnology) and equivalent amount of 20 μg protein samples were separated with a 4-12% Bis-Tris gel (Beyotime Institute of Biotechnology) and transferred onto a PVDF membrane. .. The membrane was blocked with 5% non-fat milk in TBS-Tween-20 for 1 h at room temperature, incubated with the indicated primary antibodies overnight at 4 ̊C, and then with horseradish peroxidase-conjugated secondary antibodies (1:5,000; ProteinTech Group, Inc.) at room temperature for 1 h. The primary antibodies used were specific for CSN1 (1:2,000; cat. no. 11709-1-AP; ProteinTech Group, Inc.), cyclin A2 (1:2,000, cat. no. GTX103042; GeneTex, Inc.), CDK-2 (1:2,000; cat. no. 10122-1-AP; ProteinTech Group, Inc.), CDK-4 (1:2,000; cat. no. 11026-1-AP; ProteinTech Group, Inc.), phospho (p)-CDK4 (1:2,000, cat. no. GTX00778; GeneTex, Inc.) or GAPDH (1:5,000; cat. no. 10494-1-AP; ProteinTech Group, Inc.). .. HRP‐conjugated Affinipure goat anti‐mouse IgG (H+L) (1:5,000; cat. no. SA00001-1; ProteinTech Group, Inc.) and HRP‐conjugated AffiniPure goat anti-rabbit IgG (H+L) (1:5,000; cat. no. SA00001-2; ProteinTech Group, Inc.) were used as secondary antibodies.

    Incubation:

    Article Title: CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.
    Article Snippet: Total protein was quantified using a BCA Protein Assay kit (cat. no. P0012; Beyotime Institute of Biotechnology) and equivalent amount of 20 μg protein samples were separated with a 4-12% Bis-Tris gel (Beyotime Institute of Biotechnology) and transferred onto a PVDF membrane. .. The membrane was blocked with 5% non-fat milk in TBS-Tween-20 for 1 h at room temperature, incubated with the indicated primary antibodies overnight at 4 ̊C, and then with horseradish peroxidase-conjugated secondary antibodies (1:5,000; ProteinTech Group, Inc.) at room temperature for 1 h. The primary antibodies used were specific for CSN1 (1:2,000; cat. no. 11709-1-AP; ProteinTech Group, Inc.), cyclin A2 (1:2,000, cat. no. GTX103042; GeneTex, Inc.), CDK-2 (1:2,000; cat. no. 10122-1-AP; ProteinTech Group, Inc.), CDK-4 (1:2,000; cat. no. 11026-1-AP; ProteinTech Group, Inc.), phospho (p)-CDK4 (1:2,000, cat. no. GTX00778; GeneTex, Inc.) or GAPDH (1:5,000; cat. no. 10494-1-AP; ProteinTech Group, Inc.). .. HRP‐conjugated Affinipure goat anti‐mouse IgG (H+L) (1:5,000; cat. no. SA00001-1; ProteinTech Group, Inc.) and HRP‐conjugated AffiniPure goat anti-rabbit IgG (H+L) (1:5,000; cat. no. SA00001-2; ProteinTech Group, Inc.) were used as secondary antibodies.

    Article Title: Purpurogallin carboxylic acid exhibits synergistic effects with 5‑fluorouracil on liver cancer cells in vitro by targeting ABCG2
    Article Snippet: .. Following blocking with 8% skim milk powder in TBS-Tween-20 (0.1%) (TBST) for 2 h at 28 ̊C, the membranes were incubated with primary antibodies against cyclin-dependent kinase (CDK) 4 (1:2,000; cat no. 11026-1-AP), CDK6 (1:1,000; cat no. 14052-1-AP), ABCG2 (1:1,000; cat no. 27286-1-AP) and GAPDH (1:50,000; cat no. 60004-1-Ig) all from Proteintech Group, Inc. for 16 h at 4 ̊C. .. After washing free antibodies three times with TBST, the membranes were incubated for 2 h at 28 ̊C with the corresponding HRP-conjugated Affinipure Goat Anti-Mouse (1:3,000; cat no. SA00001-1) or HRP-conjugated Affinipure Goat Anti-Rabbit (1:3,000; cat no. SA00001-2) all from Proteintech Group, Inc. secondary antibodies.

    Blocking Assay:

    Article Title: Purpurogallin carboxylic acid exhibits synergistic effects with 5‑fluorouracil on liver cancer cells in vitro by targeting ABCG2
    Article Snippet: .. Following blocking with 8% skim milk powder in TBS-Tween-20 (0.1%) (TBST) for 2 h at 28 ̊C, the membranes were incubated with primary antibodies against cyclin-dependent kinase (CDK) 4 (1:2,000; cat no. 11026-1-AP), CDK6 (1:1,000; cat no. 14052-1-AP), ABCG2 (1:1,000; cat no. 27286-1-AP) and GAPDH (1:50,000; cat no. 60004-1-Ig) all from Proteintech Group, Inc. for 16 h at 4 ̊C. .. After washing free antibodies three times with TBST, the membranes were incubated for 2 h at 28 ̊C with the corresponding HRP-conjugated Affinipure Goat Anti-Mouse (1:3,000; cat no. SA00001-1) or HRP-conjugated Affinipure Goat Anti-Rabbit (1:3,000; cat no. SA00001-2) all from Proteintech Group, Inc. secondary antibodies.



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    Silencing of ATG10 inhibited cell proliferation through cyclin B1/CDK1 and CDK2. A, B. ATG10 expression in HCC tissues and cells; C, D. Generation of ATG10-knockdown cells by siRNA; E, F. Cell proliferation as determined by CCK-8 assay; G. The expression levels of cyclin B1, CDK1 and CDK2 in ATG10-knockdown cells.

    Journal: American Journal of Cancer Research

    Article Title: ATG10 overexpression is related to the dismal prognosis and promotes the growth and migration of hepatocellular carcinoma cells via cyclin B1/CDK1 and CDK2

    doi:

    Figure Lengend Snippet: Silencing of ATG10 inhibited cell proliferation through cyclin B1/CDK1 and CDK2. A, B. ATG10 expression in HCC tissues and cells; C, D. Generation of ATG10-knockdown cells by siRNA; E, F. Cell proliferation as determined by CCK-8 assay; G. The expression levels of cyclin B1, CDK1 and CDK2 in ATG10-knockdown cells.

    Article Snippet: The primary antibodies used in this study were: ATG10 (1:1000, BOSTER, Wuhan, China), Cyclin B1 (1:1000, BOSTER, Wuhan, China), cyclin-dependent kinase (CDK) 1 (1:1000, BOSTER, Wuhan, China), CDK2 (1:1000, BOSTER, Wuhan, China), as well as β-actin (1:3000, BOSTER, Wuhan, China).

    Techniques: Expressing, Knockdown, CCK-8 Assay